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kromega Select |
 The
new kromega Select
range of HPLC columns use more than just another
new generation of HPLC packing material. The packing material is manufactured
using an entirely new process based on well established,
tried and tested silica production techniques coupled
with new refining technologies which ultimately
leads to a superior material.
Specification
The fact that high purity kromega
Select packing is manufactured using well
established silica engineering techniques means
that a wide range of specifications are possible.
As a result, the range accomodates pore sizes ranging
from 60Å to 1000Å (most popular being
120Å).
Moreover, kromega
Select is also available in particle sizes
ranging from 3µm up to preparative grade sizes.
kromega
Select ODS-H HPLC Columns
ODS-H is the most generally applicable
of the kromega Select
phases, with a carbon loading of 18%, and can be
used for both specialist applications involving
acid and basic compounds or mixtures of both. ODS-H
is also particularly well suited to general purpose
method development.
| ODS-H Example 1. |
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The absence of active silanols
in kromega Select is illustrated by this standard test mixture which includes both
pyridine and phenol. The lack of peak tailing clearly demonstrates the absence
of metallic impurities. |
1. Pyridine, 2.
Phenol |
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| COLUMN |
kromega Select ODS-H 5µm
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| DIMENSIONS |
15cm x 4.6mm |
| MOBILE PHASE |
40:60 Acetonitrile:Water |
| TEMPERATURE |
40°C |
| FLOW RATE |
1 ml/min |
| DETECTION |
UV at 254nm |
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| ODS-H Example 2. |
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Neutral silanol groups can still
occasionally cause problems with certain classes of compound. Theobromine,
theophylline and caffeine are particularly prone to this effect. In kromega Select
this effect is elimated by full end capping. |
1. Theobromine,
2. Theophylline,
3. Caffeine, 4.
Phenol |
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| COLUMN |
kromega Select ODS-H 5µm
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| DIMENSIONS |
15cm x 4.6mm |
| MOBILE PHASE |
25:75 Methanol:Water |
| TEMPERATURE |
30°C |
| FLOW RATE |
1 ml/min |
| DETECTION |
UV at 254nm |
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| ODS-H Example 3. |
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Many compounds such as polyphenols
interact irreversibly with the active sites on silica based reversed phase
materials. In the case of kromega Select ODS-H this problem does not occur, which
makes it possible to analyse problematic compounds such as in the ODS-H Example
below. |
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| COLUMN |
kromega Select ODS-H 5µm
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| DIMENSIONS |
15cm x 4.6mm |
| MOBILE PHASE |
THF:Water:Formic Acid (35:60:5) |
| TEMPERATURE |
40°C |
| FLOW RATE |
0.65 Sl/min |
| DETECTION |
UV at 254nm |
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kromega
Select ODS-L HPLC Columns
ODS-L has a slightly lower carbon
loading (15%) than the ODS-H and is useful for the
analysis of a wide range of hydrophilic compounds
which are normally not retained by other C18 based
packing material.
kromega
Select ODS-L is also as useful for analyzing
hydrophobic samples which are currently chromatographed
on competitor C8 materials. Like ODS-H, this packing
material is also fully end-capped. ODS-L is also
based on the same high purity silica as the kromega
Select ODS-H material which ensures the high
degree of inertness and lack of peak tailing.
| ODS-L Example 1. |
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One particular advantage is that
even pure water can be used as the eluent without the need for any organic
modifier. Highly polar samples only soluble in water may be separated by
this packing material, e.g. sugars and other saccharides. |
1. Sucrose,
2. Kestose
3. Fructotriose, 4.
Fructotetraose
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| COLUMN |
kromega Select ODS-L 5µm
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| DIMENSIONS |
15cm x 4.6mm |
| MOBILE PHASE |
100% Water (HPLC Grade) |
| TEMPERATURE |
40°C |
| FLOW RATE |
1 ml/min |
| DETECTION |
Refractive Index |
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Other
kromega Select Phases
We also supply other phase types:
Silica, C1, C4, C8, CN, NH2, Phenyl and Diol. To
find the right column for you, please use our finder
tool below, or contact us with your requirements: |
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| © 2012 | Company
Info
