Troubleshooting Process Chart
– Probable Instrument Fault
– Correct / Change Set-up & Re-run Analysis
– Probable Methodology Fault
– Leak in System – Pump Fault
– Detector Fault or Injector Fault
– Pump Fault or Column Compartment Fault
– Autosampler Fault
– Improve Method
– High Back Pressure Issues
– Run a Known Method on the System
– Look for Differences Between Systems
– Probable Instrument Fault
– Correct / Change Set-up & Re-run Analysis
– Probable Methodology Fault
– Leak in System – Pump Fault
– Detector Fault or Injector Fault
– Pump Fault or Column Compartment Fault
– Autosampler Fault
– Improve Method
– High Back Pressure Issues
– Run a Known Method on the System
– Look for Differences Between Systems
Autosampler Fault
Poor area reproducibility is caused by varying amounts of sample being injected. Possible causes of failure:- Worn syringe
- Sample carryover (needle seat)
- Metering device
- Faulty / worn switching valve
- Leaks inside the autosampler (e.g. Alliance autosampler leaks are not always immediately apparent)
- Seal leaks – some are more visible than others
- Leaks on the sampling side do not always show pressure changes
