HPLC Columns

kromega Select

The kromega Select range of HPLC columns use more than just another new generation of HPLC packing material. The packing material is manufactured using an entirely new process based on well established, tried and tested silica production techniques coupled with new refining technologies which ultimately leads to a superior material.

Specification

The fact that high purity kromega Select packing is manufactured using well established silica engineering techniques means that a wide range of specifications are possible. As a result, the range accomodates pore sizes ranging from 60Å to 1000Å (most popular being 120Å).
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ODS-H is the most generally applicable of the kromega Select phases, with a carbon loading of 18%, and can be used for both specialist applications involving acid and basic compounds or mixtures of both. ODS-H is also particularly well suited to general purpose method development.
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ODS-H Example 1.
	The absence of active silanols in kromega Select is illustrated by this standard test mixture which includes both pyridine and phenol. The lack of peak tailing clearly demonstrates the absence of metallic impurities.
1. Pyridine, 2. Phenol
COLUMN	kromega Select ODS-H 5µm
DIMENSIONS	15cm x 4.6mm
MOBILE PHASE	40:60 Acetonitrile:Water
TEMPERATURE	40°C
FLOW RATE	1 ml/min
DETECTION	UV at 254nm
ODS-H Example 2
	Neutral silanol groups can still occasionally cause problems with certain classes of compound. Theobromine, theophylline and caffeine are particularly prone to this effect. In kromega Select this effect is elimated by full end capping.
1. Theobromine, 2. Theophylline, 3. Caffeine, 4. Phenol
COLUMN	kromega Select ODS-H 5µm
DIMENSIONS	15cm x 4.6mm
MOBILE PHASE	25:75 Methanol:Water
TEMPERATURE	30°C
FLOW RATE	1 ml/min
DETECTION	UV at 254nm
ODS-H Example 3.
	Many compounds such as polyphenols interact irreversibly with the active sites on silica based reversed phase materials. In the case of kromega Select ODS-H this problem does not occur, which makes it possible to analyse problematic compounds such as in the ODS-H Example below.
COLUMN	kromega Select ODS-H 5µm
DIMENSIONS	15cm x 4.6mm
MOBILE PHASE	THF:Water:Formic Acid (35:60:5)
TEMPERATURE	40°C
FLOW RATE	0.65 Sl/min
DETECTION	UV at 254nm

ODS-L has a slightly lower carbon loading (15%) than the ODS-H and is useful for the analysis of a wide range of hydrophilic compounds which are normally not retained by other C18 based packing material.

kromega Select ODS-L is also as useful for analyzing hydrophobic samples which are currently chromatographed on competitor C8 materials. Like ODS-H, this packing material is also fully end-capped. ODS-L is also based on the same high purity silica as the kromega Select ODS-H material which ensures the high degree of inertness and lack of peak tailing.
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ODS-L Example 1.
	One particular advantage is that even pure water can be used as the eluent without the need for any organic modifier. Highly polar samples only soluble in water may be separated by this packing material, e.g. sugars and other saccharides.
1. Pyridine, 2. Phenol
COLUMN	kromega Select ODS-L 5µm
DIMENSIONS	15cm x 4.6mm
MOBILE PHASE	100% Water (HPLC Grade)
TEMPERATURE	40°C
FLOW RATE	1 ml/min
DETECTION	Refractive Index

We also supply other phase types: Silica, C1, C4, C8, CN, NH2, Phenyl and Diol. To find the right column for you, please select your phase requirement below, and then filter: