- Probable Instrument Fault
- Correct / Change Set-up & Re-run Analysis
- Probable Methodology Fault
- Leak in System - Pump Fault
- Detector Fault or Injector Fault
- Pump Fault or Column Compartment Fault
- Autosampler Fault
- Improve Method
- High Back Pressure Issues
- Run a Known Method on the System
- Look for Differences Between Systems
High Back Pressure Issues
High back pressure is indicative of a blockage in the system.
Check flow, tubing and column. Start at the detector and work towards the pump to identify the location of the blockage.
PEEK fitting can fail if tubing is necked, kinked.
Remove tubing from column outlet – is there flow through the column?
Pressure sensor could also be faulty.