Look for Differences Between Systems

We mean:

  • Is it the same make?
  • Is it the same model (exactly)?
  • Has it got the same configuration?

For example:

  • If one has a quaternary and one has a binary pump, the gradient profile may be different as they use low and high pressure mixing respectively.
  • DAD vs VWD – both use UV detection but sensitivity will be different.
  • Syringe vs Metering Drive
  • Data capture rate setting on software.
  • Tubing connecting components can be a crucial part of the system.

Any differences can/will have an effect on the chrmoatography.