- Probable Instrument Fault
- Correct / Change Set-up & Re-run Analysis
- Probable Methodology Fault
- Leak in System - Pump Fault
- Detector Fault or Injector Fault
- Pump Fault or Column Compartment Fault
- Autosampler Fault
- Improve Method
- High Back Pressure Issues
- Run a Known Method on the System
- Look for Differences Between Systems
Step 2 – Look for the obvious first
Most faults are derived from simple issues. If the initial inspection of the system hasn’t shown an obvious fault, check the following:
- Has the correct method been run?
- Could the method have been overwritten / changed?
- Were the samples, standards and mobile phases prepared correctly?
- Is the sequence correct?
Look at the preparation records for standards, samples and mobile phases, i.e. is the mobile phase 70/30 not 30/70?