- Probable Instrument Fault
- Correct / Change Set-up & Re-run Analysis
- Probable Methodology Fault
- Leak in System - Pump Fault
- Detector Fault or Injector Fault
- Pump Fault or Column Compartment Fault
- Autosampler Fault
- Improve Method
- High Back Pressure Issues
- Run a Known Method on the System
- Look for Differences Between Systems
Probable Methodology Fault
- ruled out user error
- used a known method to evaluate the systems performance
but are still getting variable results; this indicates a problem with the method robustness.
If the method works intermittently, it is likely that it is just a matter of “balancing” the methodology, for example:
- the mobile phase pH is not quite right
- the column compartment may be too hot (or cold)
- the gradient may not have enough / too much organic solvent
Look at the injection size. Are you near / over the capacity of the column?
Still not getting anywhere, why not call our technical support team for advice on 01227 265333.