Probable Methodology Fault

You have:

  • ruled out user error
  • used a known method to evaluate the systems performance

but are still getting variable results; this indicates a problem with the method robustness.

If the method works intermittently, it is likely that it is just a matter of “balancing” the methodology, for example:

  • the mobile phase pH is not quite right
  • the column compartment may be too hot (or cold)
  • the gradient may not have enough / too much organic solvent

Look at the injection size. Are you near / over the capacity of the column?

Still not getting anywhere, why not call our technical support team for advice on 01227 265333.