Step 6 >>

Step 5 -Investigation tests

If there is no data to determine instrument performance the following tests can used for investigation purposes:

  • Repeatability – run 5-10 injections from a single vial (retention time and area should be repeatable)
  • Monitor pressure – high pressure may indicate a blockage, low pressure a leak
  • Check the gradient valve composition (UV Absorbance is linear therefore the absorbance at 50% should be 50% of the Absorbance at 100%).
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