Troubleshooting Process Chart
– Probable Instrument Fault
– Correct / Change Set-up & Re-run Analysis
– Probable Methodology Fault
– Leak in System – Pump Fault
– Detector Fault or Injector Fault
– Pump Fault or Column Compartment Fault
– Autosampler Fault
– Improve Method
– High Back Pressure Issues
– Run a Known Method on the System
– Look for Differences Between Systems
– Probable Instrument Fault
– Correct / Change Set-up & Re-run Analysis
– Probable Methodology Fault
– Leak in System – Pump Fault
– Detector Fault or Injector Fault
– Pump Fault or Column Compartment Fault
– Autosampler Fault
– Improve Method
– High Back Pressure Issues
– Run a Known Method on the System
– Look for Differences Between Systems
Step 5 -Investigation tests
If there is no data to determine instrument performance the following tests can used for investigation purposes:- Repeatability – run 5-10 injections from a single vial (retention time and area should be repeatable)
- Monitor pressure – high pressure may indicate a blockage, low pressure a leak
- Check the gradient valve composition (UV Absorbance is linear therefore the absorbance at 50% should be 50% of the Absorbance at 100%).
