Troubleshooting Process Chart
- Probable Instrument Fault
- Correct / Change Set-up & Re-run Analysis
- Probable Methodology Fault
- Leak in System - Pump Fault
- Detector Fault or Injector Fault
- Pump Fault or Column Compartment Fault
- Autosampler Fault
- Improve Method
- High Back Pressure Issues
- Run a Known Method on the System
- Look for Differences Between Systems
- Probable Instrument Fault
- Correct / Change Set-up & Re-run Analysis
- Probable Methodology Fault
- Leak in System - Pump Fault
- Detector Fault or Injector Fault
- Pump Fault or Column Compartment Fault
- Autosampler Fault
- Improve Method
- High Back Pressure Issues
- Run a Known Method on the System
- Look for Differences Between Systems
Step 5 -Investigation tests
If there is no data to determine instrument performance the following tests can used for investigation purposes:
- Repeatability – run 5-10 injections from a single vial (retention time and area should be repeatable)
- Monitor pressure – high pressure may indicate a blockage, low pressure a leak
- Check the gradient valve composition (UV Absorbance is linear therefore the absorbance at 50% should be 50% of the Absorbance at 100%).
